Kusvibiswa kwetsika dzemasero kunogona kuve nyore nyore dambudziko rinowanzoitika mumarabhoritari etsika, dzimwe nguva zvichikonzera mhedzisiro yakakomba.Sero tsika zvinosvibisa zvinogona kupatsanurwa muzvikamu zviviri, makemikari zvinosvibisa akadai svikiro, serum uye mvura tsvina, endotoxins, plasticizers uye detergents, uye zvipenyu zvinosvibisa zvakadai mabhakitiriya, molds, mbiriso, mavhairasi, mycoplasmas muchinjikwa utachiona.Yakasvibiswa nemamwe mitsara yesero.Kunyangwe zvisingaite kubvisa zvachose kusvibiswa, kuwanda kwayo uye kuomarara kunogona kudzikiswa nekunyatsonzwisisa kwainotangira uye nekutevera yakanaka aseptic maitiro.
1.Chikamu ichi chinodonongodza mhando huru dzekusvibiswa kwebiological:
Kusvibiswa kwebhakitiriya
Mold uye kusvibiswa kwehutachiona
Mycoplasma kusvibiswa
Kusvibiswa kwembiriso
1.1Kusvibiswa kwebhakitiriya
Utachiona iboka guru rezvipembenene zvinowanika kwose kwose.Anowanzo angori mamicrons mashoma muhupamhi uye anogona kuuya mumhando dzakasiyana-siyana, kubva kubhora kuenda kune tsvimbo uye spirals.Nekuda kwekuwanda kwavo, saizi, uye kukurumidza kukura kwekukura, mabhakitiriya, pamwe nembiriso uye mold, ndiwo anonyanya kusvibisa zvipenyu mumasero tsika.
1.1.1 Kuonekwa kweKusvibiswa kweBhakitiriya
Kusvibiswa kwebhakitiriya kunowanikwa nyore nyore nekutarisa kwekuona kwetsika mukati memazuva mashomanana kubva pakuita hutachiona;
Tsika dzine utachiona dzinowanzoita sedzakasviba (kureva, turbid), dzimwe nguva paine firimu rakatetepa pamusoro.
Kamwe kamwe madonhwe mu pH yetsika yepakati anowanzo sangana.
Pasi pemaikorosikopu ine simba rakaderera, utachiona hunoratidzika sediki, hunofamba-famba pakati pemasero, uye kutarisa pasi pemakorosikopu ane simba guru anogona kugadzirisa maumbirwo ebhakitiriya ega.
1.2Mold & Virus kusvibiswa
1.2.1 Kusvibiswa kweMold
Molds maeukaryotic microorganisms ehumambo hwefungal inokura muchimiro chemulticellular filaments inonzi hyphae.Manetiweki anosanganisirwa eaya mafilamenti akawanda ane genetically yakafanana nuclei inonzi colonies kana mycelium.
Zvakafanana nekusvibiswa kwembiriso, iyo pH yetsika inoramba yakagadzikana panguva yekutanga kusvibiswa uye yobva yawedzera nekukurumidza sezvo tsika inotapukirwa zvakanyanya uye ichiita makore.Pasi pemaikorosikopu, mycelium inowanzoita filamentous, dzimwe nguva semasumbu akaonda espores.Maspores emhando dzakawanda anogona kurarama munzvimbo dzakaomarara uye dzisina mutsa panguva yekurara uye anovhurwa chete kana mamiriro ekukura akasangana.
1.2.2 Kusvibiswa kweVirus
Mavhairasi imicroscopic infectious agents anotora mashandiro emuchina wesero rekuberekesa.Saizi yavo idiki zvakanyanya inoita kuti zviome kuona mutsika uye kubvisa kubva kune mareagents anoshandiswa mu cell tsika marabhoritari.Sezvo mavhairasi mazhinji aine zvinodiwa zvakanyanya kune avo vanoagamuchira, kazhinji haakanganisa maitiro emasero emarudzi kunze kweanotambira.
Nekudaro, kushandiswa kwemasero ane hutachiona hwetsika kunogona kuisa njodzi kuhutano hwevashandi vemurabhoritari, kunyanya kana maseru emunhu kana eprimate akakura murabhoritari.
Utachiona hwehutachiona mumasero hunogona kuonekwa neelectron microscopy, immunostaining neseti yemasoja ekudzivirira chirwere, ELISA, kana PCR ine mavhairasi akakodzera.
1.3Kusvibiswa kweMycoplasma
Mycoplasmas mabhakitiriya ari nyore asina madziro emasero, uye anofungidzirwa kuti ndiwo tupukanana anozvidzokorora.Nekuda kwehukuru hwadzo hudiki (kazhinji isingasviki 1 micron), mycoplasma inonetsa kuona kusvika yasvika pakunyanya kusimba uye kuita kuti tsika dzemasero dzive dzakaipa;Kusvikira panguva iyoyo, kazhinji hapana chiratidzo chiri pachena chechirwere.
1.3.1 Kuonekwa kwekusvibiswa kwemycoplasma
Mamwe mamycoplasmas anononoka kukura anogona kuramba ari mutsika pasina kukonzera kufa kwesero, asi anoshandura maitiro uye metabolism yemasero anotambira mutsika.
Chronic mycoplasma chirwere chinogona kuratidzirwa nekuderedzwa kwesero kuwedzera kwehuwandu, kuderera kwehuwandu hwehuwandu uye agglutination mukusimisa tsika.
Zvisinei, nzira chete yakavimbika yekuona mycoplasma kusvibiswa ndeyekuedza tsika nguva dzose uchishandisa fluorescent staining (eg, Hoechst 33258), ELISA, PCR, immunostaining, autoradiography, kana microbial test.
1.4Kusvibiswa kwembiriso
Yeasts i-single-celled eukaryotes yehumambo hwefungal, kubva muhukuru kubva kune mashoma mamicrons (kazhinji) kusvika ku40 microns (kashoma).
1.4.1Kuonekwa kwekusvibiswa kwembiriso
Sekusvibiswa kwebhakitiriya, tsika dzakasvibiswa nembiriso dzinogona kusviba, kunyanya kana kusvibiswa kuri padanho repamusoro.Iyo pH yetsika yakasvibiswa nembiriso inoshanduka zvishoma kusvika kusvibiswa kwawedzera, panguva iyo pH inowanzowedzera.Pasi pemaikorosikopu, mbiriso inoratidzika seyega yega ovoid kana spherical particles uye inogona kuburitsa zvidimbu zvidiki.
2.Cross chirwere
Kunyange zvazvo zvisingawanzoitiki sekusvibiswa kwehutachiona, kusvibiswa kwakawanda kwemuchinjiko wemasero akawanda ane HeLa nemamwe mitsara inokura nokukurumidza idambudziko rinonyatsotsanangurwa rine migumisiro yakakomba.Tora mitsara yesero kubva kune anoremekedzwa maseru mabhangi, gara uchitarisa maitiro emasero mitsara, uye shandisa yakanaka aseptic matekiniki.Aya maitiro anozokubatsira kuti urege kusvibiswa.DNA kudhindisa zvigunwe, karyotyping uye isotyping inogona kusimbisa kana paine kuchinjika-kusvibiswa mune yako sero tsika.
Kunyange zvazvo zvisingawanzoitiki sekusvibiswa kwehutachiona, kusvibiswa kwakawanda kwemuchinjiko wemasero akawanda ane HeLa nemamwe mitsara inokura nokukurumidza idambudziko rinonyatsotsanangurwa rine migumisiro yakakomba.Tora mitsara yesero kubva kune anoremekedzwa maseru mabhangi, gara uchitarisa maitiro emasero mitsara, uye shandisa yakanaka aseptic matekiniki.Aya maitiro anozokubatsira kuti urege kusvibiswa.DNA kudhindisa zvigunwe, karyotyping uye isotyping inogona kusimbisa kana paine kuchinjika-kusvibiswa mune yako sero tsika.
Nguva yekutumira: Feb-01-2023