Saiti-Yakasiyana Kubatanidzwa Isa Zvakananga Iyo Target Genes muSpecific Hot Spot
Saiti-chaiyo kubatanidzwa imaitiro ekugadzirisa webhusaiti kana application kuti ikwane izvo zvakasarudzika zveimwe webhusaiti kana application.Iyo inzira inosanganisira kuita shanduko kune iripo kodhi uye chimiro chewebhusaiti kana application kuitira kuti iite kuti ishande uye ishande kune chaiyo zvinodiwa zvesaiti.Saiti-chaiyo yekubatanidza inogona kushandiswa kugadzirisa zviripo, kuwedzera maficha matsva, uye kunatsiridza kushandiswa kwese kwesaiti kana application.Izvi zvinonyanya kubatsira kune mabhizinesi ane akawanda mawebhusaiti kana maapplication anoda kubatanidzwa kune mumwe nemumwe kuitira kupa vatengi vavo ruzivo rusina musono.
Saiti-yakananga kubatanidzwa muCHO masero inzira inoshandiswa kuunza gene rekufarira munzvimbo yakanyatsotsanangurwa mune genome yeChinese hamster ovary (CHO) masero.Kuita uku kunosanganisira kushandisa saiti-chaiyo recombinase enzyme kuti itarise yakatarwa kutevedzana muCHO cell genome uyezve kubatanidza gene rekufarira mune yakanangwa kutevedzana.Iyi nzira inobvumira kudzora kwakaringana pamusoro pekuiswa kwemajini muCHO cell genome uye inogona kubatsira kudzivirira kusanganisa zvisina tsarukano, izvo zvinogona kuva nemhedzisiro yakaipa pamaseru.Kubatsira kukuru kweiyi nzira ndeyokuti inopa kutonga kukuru uye kururamisa pamusoro pekubatanidza maitiro, pamwe chete nehuwandu hwekugadzikana kwejeni nekufamba kwenguva.Pamusoro pezvo, nzira iyi inogona kushandiswa kuunza majini akawanda munzvimbo dzakasiyana mukati mesero, ichiita chishandiso chine simba chekugadzirisa majini.
Targeting vectors
Targeting vectors anoshandiswa kugadzira zvipenyu zvakagadziridzwa genetically kuunza chaiyo DNA sequences muma genomes avo.Iwo anowanzo kuumbwa nemajini mamakisi anobvumira kuzivikanwa kweakagadziridzwa maseru, inosarudzika mucherechedzo unobvumira kusarudzwa kwemasero akagadziridzwa, uye homologous recombination dunhu rinobvumira kubatanidzwa kweinodiwa DNA kutevedzana mugenome yechinhu chinotarisirwa.Targeting vectors anowanzo shandiswa mukugogodza gene, gene knockins, gene editing, uye mamwe maitiro emajini engineering.
