1.Chii chinonzi sero tsika?
Cell Culture zvinoreva kubvisa maseru kubva kumhuka kana zvirimwa wobva waakurisa munzvimbo yakanaka yekugadzira.Iwo maseru anogona kutorwa zvakananga kubva munyama uye akaputswa ne enzymatic kana mechanic nzira asati agadzira, kana anogona kutorwa kubva kune akamiswa maseru mitsara kana maseru mitsara.
2.Chii chinonzi tsika yekutanga?
Tsika yekutanga inoreva nhanho yetsika mushure mekunge masero aparadzaniswa kubva munyama uye anowedzera pasi pemamiriro akakodzera kudzamara atora ese anowanikwa substrates (kureva, kusvika pakusangana).Panguva ino, maseru anofanirwa kuve akaumbwa nekuaendesa kumudziyo mutsva une svikiro rekukura kuti ripe imwe nzvimbo yekuenderera mberi nekukura.
2.1 Cell line
Mushure mekutanga subculture, tsika yekutanga inonzi cell line kana subclone.Mitsara yemasero anobva kutsika dzekutanga ane nguva shoma yekurarama (kureva kuti ishoma; ona pazasi), uye sezvaanodarika, maseru ane simba rekukura repamusoro anotonga, zvichikonzera imwe dhigirii ye genotype muhuwandu inopokana ne phenotype.
2.2 Kunetsa kwesero
Kana iyo subpopulation yesero mutsara yakasarudzwa zvakanaka kubva kutsika ne cloning kana imwe nzira, iyo sero mutsara inozoita sero dhizaini.Masero emhando anowanzo kuwana mamwe mageneti ekuchinja mushure mekunge mutsara wevabereki watanga.
3.Limited uye inopfuurira sero mitsara
Masero akajairika anowanzo patsanura nhamba shoma yenguva asati arasikirwa nekukwanisa kuwanda.Ichi chiitiko chakatemwa nemajini chinonzi senescence;mitsara yesero iyi inonzi finite cell lines.Zvakadaro, mamwe mitsara yesero inove isingafi kuburikidza nemaitiro anonzi shanduko, ayo anogona kuitika ega kana anogona kukonzerwa nemakemikari kana mavhairasi.Kana mutsara wesero ukapinda shanduko uye wowana kugona kupatsanura nekusingaperi, inove inoenderera sero mutsara.
4.Culture mamiriro
Mamiriro etsika erudzi rwesero akasiyana zvakanyanya, asi nharaunda yekugadzira masero anogara akaumbwa nemudziyo wakakodzera, une zvinotevera:
4.1 Substrate kana tsika yepakati inopa zvakakosha zvinovaka muviri (amino acids, makabhohaidhiretsi, mavhitaminzi, zvicherwa)
4.2 Zvinhu zvinokura
4.3 Mahomoni
4.4 Magasi (O2, CO2)
4.5 Yakadzorwa yemuviri uye makemikari nharaunda (pH, osmotic pressure, tembiricha)
Masero mazhinji anotsamira pa anchorage uye anofanirwa kusimudzwa pane yakasimba kana semi-solid substrate (inonamatira kana monolayer tsika), nepo mamwe masero anogona kukura achiyangarara pakati (yakarembera tsika).
5.Cryopreservation
Kana pane masero akawandisa mu-subculture, anofanirwa kurapwa neakakodzera kudzivirira agent (akadai seDMSO kana glycerol) uye akachengetwa pakupisa pasi -130 ° C (cryopreservation) kusvikira yava kudiwa.Kuti uwane rumwe ruzivo nezve subculture uye cryopreservation yemasero.
6.Morphology yemasero mutsika
Masero mutsika anogona kupatsanurwa muzvikamu zvitatu zvakakosha zvichienderana nechimiro uye chimiro chavo (kureva morphology).
6.1 Masero eFibroblasts ane bipolar kana multipolar, ane chimiro chakarebesa, uye anokura akabatanidzwa kune substrate.
6.2 Epithelial-like maseru ane polygonal, ane saizi yakajairwa, uye anonamirwa kune matrix mune discrete sheet.
6.3 Masero akaita seLymphoblast ane denderedzwa uye anowanzokura ari mukumiswa asina kunamatira kumusoro.
7.Kushandiswa kwesero tsika
Cell tsika ndechimwe chezvishandiso zvikuru zvinoshandiswa musero uye molecular biology.Inopa yakanakisa modhi sisitimu yekudzidza yakajairwa physiology uye biochemistry yemasero (sekutsvagisa metabolic, kuchembera), mhedzisiro yezvinodhaka uye zvine chepfu pamasero, uye mutagenesis uye carcinogenic mhedzisiro.Inoshandiswawo pakuongorora zvinodhaka uye kusimudzira uye kugadzirwa kwakakura kwebiological compounds (senge majekiseni, mapuroteni ekurapa).Mukana mukuru wekushandisa sero tsika kune chero yeaya maapplication ndiko kuenderana uye kuberekana kwemhedzisiro inogona kuwanikwa uchishandisa batch yeakaumbwa maseru.
Nguva yekutumira: Jun-03-2019