1.Kusarudza mutsara wesero wakarurama
Paunenge uchisarudza iyo yakakodzera cell line yekuyedza kwako, ndapota funga zvinotevera maitiro:
a.Spees: Mitsara yemasero asiri emunhu neasiri eprimate kazhinji ane zvirambidzo zvishoma zvekuchengetedzwa kwezvipenyu, asi pakupedzisira kuedza kwako kuchasarudza kana kushandisa tsika yerudzi rwakati.
b.Zvinhu: Chinangwa chekuedza kwako ndechei?Semuenzaniso, mitsara yesero inotorwa kubva pachiropa neitsvo inogona kuve yakakodzera kuongororwa kwehupfu.
c.Inogumira kana kuenderera mberi: Kunyangwe kusarudza kubva kune diki mutsara wesero kunogona kukupa dzimwe sarudzo dzekuratidza basa chairo, inoenderera mitsara yesero inowanzove nyore kuumba nekuchengetedza.
d.Normal kana kushandurwa: Mitsara yemasero yakashandurwa inowanzova nekukura kwekukura kwepamusoro uye kubudirira kwembeu yepamusoro, inoenderera mberi, uye inoda serum shoma mumutauro wetsika, asi phenotype yavo yakachinja zvachose kuburikidza nekuchinja kwemajini.
e. Mamiriro ekukura uye maitiro: Ndezvipi zvaunoda kuti uwedzere kukurumidza, saturation density, cloning kunyatsoshanda uye kugona kukura kwekurembera?Semuenzaniso, kuratidza recombinant mapuroteni mugoho rakakura, ungangoda kusarudza mitsara yesero ine kukurumidza kukura kwemazinga uye kugona kukura mukurembera.
f.Mamwe maitiro: Kana uri kushandisa mutsara wesero shoma, pane stock yakakwana iripo?Mutsara wesero unonyatso zivikanwa here, kana kuti unofanira kuzviongorora wega?Kana iwe uri kushandisa abnormal cell line, pane yakaenzana yakajairika cell line inogona kushandiswa sekutonga?Iyo cell line yakagadzikana here?Kana zvisiri, zviri nyore sei kuigadzira uye kugadzira chando chakaringana chekuyedza kwako?
2.Obtain cell lines
Iwe unogona kuvaka yako tsika kubva kumaseru ekutanga, kana iwe unogona kusarudza kutenga yakasimbiswa maseru tsika kubva kune vekutengesa kana vasina-purofiti vatengesi (kureva maseru mabhanga).Vatengesi vane mukurumbira vanopa mitsara yemhando yepamusoro-soro iyo yakanyatsoedzwa kuti ione kutendeseka uye kuona kuti tsika yacho haina tsvina.Isu tinokurudzira kusakwereta tsika kubva kune mamwe marabhoritari nekuti ivo vane njodzi yakakura yekusvibiswa kwesero tsika.Zvisinei nekwairi kubva, ndapota ita shuwa kuti mitsetse yese yesero yakaongororwa kusvibiswa kwe mycoplasma usati watanga kuishandisa.
Nguva yekutumira: Feb-01-2023