Cell Culture Media Ipuratifomu Yekuvandudza Yakagadzirirwa
Cell culture media muto unovaka muviri une zvakakosha zvinovaka muviri uye kukura zvinhu zvinodiwa pakukura kwesero nekuchengetedza.Inowanzo kuumbwa musanganiswa wakaenzana wemakhahydrates, mapuroteni, lipids, zvicherwa, mavitamini, uye kukura zvinhu.Nhepfenyuro dzinopawo nharaunda yakanaka kuti maseru abudirire mukati, senge pH yakakwana, osmotic pressure, uye tembiricha.Nhepfenyuro inogonawo kuva nemishonga inorwisa mabhakitiriya kudzivirira kusvibiswa kwebhakitiriya kana fungal, uye zvimwe zvinowedzera kuwedzera kukura kwemhando dzakasiyana dzemasero.Cell culture media inoshandiswa mukutsvagisa kwakasiyana-siyana uye mashandisirwo ekurapa, akadai setishu engineering, kuwanikwa kwezvinodhaka, uye kutsvaga kenza.
Dzinde Kutengesa Tsika Media
Stem cell culture media kazhinji ine musanganiswa webasal medium, senge Dulbecco's Modified Eagle Medium (DMEM) kana RPMI-1640, uye serum supplement, senge fetal bovine serum (FBS).Iyo basal svikiro inopa zvakakosha zvinovaka muviri uye mavhitamini, nepo serum yekuwedzera inowedzera kukura zvinhu, senge insulin, transferrin uye selenium.Pamusoro pezvo, stem cell tsika media inogona kunge iine mishonga inorwisa mabhakitiriya, senge penicillin, kudzivirira kusvibiswa nebhakitiriya.Mune zvimwe zviitiko, zvimwe zvekuwedzera, senge recombinant kukura zvinhu, zvinogona kuwedzerwa kune tsika midhiya kuwedzera stem cell kukura kana kusiyanisa.
Munhu Embryonic Stem Cell
Embryonic stem cells (ESCs) ndiwo masero anotorwa kubva mukati mesero remukati reblastocyst, yekutanga-nhanho preimplantation embryo.MaESC evanhu anonzi mahESC.Iwo ari pluripotent, zvichireva kuti vanokwanisa kusiyanisa mumasero ese emhando nhatu dzekutanga hutachiona: ectoderm, endoderm uye mesoderm.Iwo chishandiso chakakosha pakudzidza budiriro biology, uye kushandiswa kwavo mukurapa kwekuvandudza kurapa zvirwere zvakasiyana-siyana kwave kuri kutariswa kwetsvakiridzo huru.
